Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2891(2)] to Angiopoietin 2/ANG2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Rabbit monoclonal [EPR2891(2)] to Angiopoietin 2/ANG2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human Angiopoietin 2/ANG2. The exact sequence is proprietary.
Database link: O15123 -
Positive control
- WB: Mouse small intestine tissue lysate - total protein (ab7939), TF1, HUVEC and ECV-304 cell lysates. Mouse spleen and rat kidney tissue lysates.
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General notes
This product was previously labelled as Angiopoietin 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2891(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)
Lane 1 : TF-1 whole cell lysate
Lane 2 : HUVEC whole cell lysate
Lane 3 : ECV-304 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/1000 dilution (unpurified)
Lane 1 : TF1 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa
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