Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13272] to Rab9 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rab9 antibody [EPR13272] - BSA and Azide free
    See all Rab9 primary antibodies

  • Description

    Rabbit monoclonal [EPR13272] to Rab9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 cells. WB: MDA-MB-231, MCF7 and HeLa cell lysates.

  • General notes

    Ab236127 is the carrier-free version of ab179815. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Western blot - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB9A knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179815).

    Lanes 1-4: Merged signal (red and green). Green - ab179815 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab179815 Anti-Rab9 antibody [EPR13272]  was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used.  Wild-type and Rab9 knockout samples were subjected to SDS-PAGE.  ab179815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescent staining of HepG2 cells labeling Rab9 with unpurified ab179815 at 1/50 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

    Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179815).

  • Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)
    Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Rab9 antibody [EPR13272] - BSA and Azide free (ab236127)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.