HRP Anti-CPT1A antibody [8F6AE9] (ab198494)
![HRP Anti-CPT1A antibody [8F6AE9] (ab198494)](https://www.abcam.com/ps/products/198/ab198494/Images/ab198494-367703-anit-cpt1a-8f6ae9-hrp-western-blot-knockout-hap1-cpt1a-hela-mcf7-human-lysates.jpg "HRP Anti-CPT1A antibody [8F6AE9] (ab198494)")
Key features and details
- HRP Mouse monoclonal [8F6AE9] to CPT1A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-CPT1A antibody [8F6AE9]
See all CPT1A primary antibodies -
Description
HRP Mouse monoclonal [8F6AE9] to CPT1A -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human CPT1A aa 450 to the C-terminus.
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Positive control
- WB: HepG2, HAP1, MCF7 and HeLa whole cell lysate. Human heart and rat liver tissue lysates. IHC-P: normal human colon tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
8F6AE9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)All lanes : HRP Anti-CPT1A antibody [8F6AE9] (ab198494) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CPT1A knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 3 minutesLanes 1 - 4: Merged signal (red and green). Green - ab198494 observed at 88 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab198494 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CPT1A knockout cells. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab198494 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)](https://www.abcam.com/ps/products/198/ab198494/Images/ab198494-242215-IHCAP22494781.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)IHC image of CPT1A staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198494 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)](https://www.abcam.com/ps/products/198/ab198494/Images/ab198494-238432-WBab1984941.jpg)
Western blot - HRP Anti-CPT1A antibody [8F6AE9] (ab198494)All lanes : HRP Anti-CPT1A antibody [8F6AE9] (ab198494) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198494 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
