Mouse sTNF RI Matched Antibody Pair Kit (ab213748)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 1.95 pg/ml - 125 pg/ml
Overview
-
Product name
Mouse sTNF RI Matched Antibody Pair Kit
See all TNF Receptor I kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.26 pg/ml -
Range
1.95 pg/ml - 125 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse sTNF RI Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse sTNF RI.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202408. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse sTNF RI Capture Antibody 2 x 50µg 1 x 50µg Mouse sTNF RI Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse sTNF RI Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
-
Alternative names
- CD120a
- FPF
- MGC19588
see all -
Database links
- Entrez Gene: 21937 Mouse
- SwissProt: P25118 Mouse
Images
-
Mouse sTNF RI standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Mouse sTNF RI Matched Antibody Pair Kit (ab213748)To learn more about the advantages of recombinant antibodies see here.
