Human Pancreatic Amylase ELISA Kit (ab137969)
Key features and details
- Sensitivity: 0.15 mU/ml
- Range: 0.313 mU/ml - 20 mU/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Pancreatic Amylase ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall = 5.6% Inter-assay Sample n Mean SD CV% Overall = 9.9% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.15 mU/ml -
Range
0.313 mU/ml - 20 mU/ml -
Recovery
> 96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Pancreatic Amylase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human pancreatic amylase in plasma, serum, urine, milk, saliva, and cell culture supernatants.
A Pancreatic Amylase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Pancreatic Amylase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Pancreatic Amylase captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Pancreatic Amylase Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Pancreatic Amylase Microplate (12 x 8 well strips) 1 unit Pancreatic Amylase Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Sequence similarities
Belongs to the glycosyl hydrolase 13 family. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- 1
- 4-alpha-D-glucan glucanohydrolase
- AMY2A
see all -
Database links
- Entrez Gene: 279 Human
- SwissProt: P04746 Human
- Unigene: 654437 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab137969
