Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-P2Y12 antibody [EPR18611] (ab184411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18611] to P2Y12
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-P2Y12 antibody [EPR18611]
    See all P2Y12 primary antibodies

  • Description

    Rabbit monoclonal [EPR18611] to P2Y12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain tissue lysates. IP: Rat brain whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)
    Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)

    P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat brain whole cell lysate 10µg (Input).
    Lane 2: ab184411 IP in Rat brain whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)
    Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)
    All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)
    Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)
    All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-P2Y12 antibody [EPR18611] (ab184411)
    Anti-P2Y12 antibody [EPR18611] (ab184411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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