Anti-CDC42 antibody [M430] (ab202280)
![Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-260582-ab202280KOWB.jpg "Anti-CDC42 antibody [M430] (ab202280)")
Key features and details
- Mouse monoclonal [M430] to CDC42
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-CDC42 antibody [M430]
See all CDC42 primary antibodies -
Description
Mouse monoclonal [M430] to CDC42 -
Host species
Mouse -
Specificity
ab202280 does not recognize a full length human RhoA GST fusion protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Human
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Immunogen
Recombinant full length protein corresponding to Rat CDC42 aa 1 to the C-terminus.
Database link: Q8CFN2 -
Positive control
- Jurkat cells and mouse brain lysates; rat A7r5 cells; recombinant full length rat Cdc42 fusion protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
M430 -
Isotype
IgM -
Research areas
Images
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Western blot - Anti-CDC42 antibody [M430] (ab202280)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab202280 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.ab202280 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab202280 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-243556-ab202280WB2.jpg)
Western blot - Anti-CDC42 antibody [M430] (ab202280)All lanes : Anti-CDC42 antibody [M430] (ab202280) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : mouse brain lysate
Lane 3 : Recombiant full length rat Cdc42 protein
Predicted band size: 21 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-243555-ab2022802.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)Immunofluorescence analysis of paraformaldehyde-fixed rat A7r5 cells, labeling CDC42 using ab202280 at a 1/200 dilution, in the absence (left, black protein block) or presence (right, red image) of full length Cdc42 recombinant protein. Goat anti-mouse conjugated to DyLight® 594 was used as secondary antibody.
