Rat Fibronectin ELISA Kit (ab108850)
Key features and details
- Sensitivity: 75 pg/ml
- Range: 0.391 ng/ml - 6.25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Fibronectin ELISA Kit
See all Fibronectin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.8% Inter-assay Sample n Mean SD CV% Overall 9.3% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
75 pg/ml -
Range
0.391 ng/ml - 6.25 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s Fibronectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin in plasma, serum, urine, and cell culture supernatants.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat Fibronectin Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Rat Fibronectin Microplate (12 x 8 well strips) 1 unit Rat Fibronectin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
Database links
- Entrez Gene: 25661 Rat
- SwissProt: P04937 Rat
- Unigene: 1604 Rat
Images
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Typical Standard Curve
Representative Standard Curve using ab108850.
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Sandwich ELISA - Ab108850 Fibronectin Rat ELISA KitFibronectin measured in various samples showing quantity (microgram) per mL of tested sample