FirePlex Mouse Kidney Toxicity - Immunoassay Panel (ab235661)
Overview
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Product name
FirePlex Mouse Kidney Toxicity - Immunoassay Panel -
Detection method
Flow cytometry-fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Multiplex -
Assay time
3h 30m -
Species reactivity
Reacts with: Mouse -
Product overview
FirePlex Mouse Kidney Toxicity - Immunoassay Panel (ab235661) contains all the reagents you need to run a 5-plex assay: antibody conjugated particles, Rodent Core Reagents and Protein Standard Mix.
NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.
Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.
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Notes
The following information provides the sensitivity, range, and required Protein Standard Mix.
Target
Sensitivity (pg/mL)
Dynamic Range (pg/mL)
Protein Standard
Clusterin
23.00
123 - 270,000
Mouse Kidney Toxicity Protein Standard Mix
Cystatin C
0.15
1.52 - 3,333
Mouse Kidney Toxicity Protein Standard Mix
KIM-1
0.07
0.51 - 1,111
Mouse Kidney Toxicity Protein Standard Mix
Lipocalin-2
0.06
0.51-1,111
Mouse Kidney Toxicity Protein Standard Mix
Osteopontin
12.29
41.1 - 90,000
Mouse Kidney Toxicity Protein Standard Mix
Negative Control Particles
N/A
N/A
N/A
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Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Kidney Toxicity Capture Particles (ab235661) 1 unit 10X Wash Buffer 1 x 25ml 15X Mouse Kidney Toxicity Biotin Detectors (ab235661) 1 unit 2X Rodent Assay Diluent 1 x 15ml 5X Reporter Solution 1 x 4ml 96-well microplate with a filter 1 unit Filter Plate Seal 1 x 3 units Mouse Kidney Toxicity Protein Standard Mix 1 unit Tungsten cleaning wire 1 unit -
Research areas
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Cellular localization
Clusterin: Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. Osteopontin: Secreted. Cystatin C: Secreted. TIM 1: Membrane. Lipocalin-2 / NGAL: Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. -
Database links
- Entrez Gene: 12759 Mouse
- Entrez Gene: 13010 Mouse
- Entrez Gene: 16819 Mouse
- Entrez Gene: 171283 Mouse
- Entrez Gene: 20750 Mouse
- SwissProt: P10923 Mouse
- SwissProt: P11672 Mouse
- SwissProt: P21460 Mouse
see all
Images
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Example of Mouse Kidney Toxicity – Immunoassay Panel standard curves.
Mean background-subtracted data values are graphed (+/- SD).
