All lanes : HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CDC42 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 21 kDa


Exposure time: 20 minutes

ab215810 was shown to recognize CDC42 in wild-type HAP1 cells as signal was lost at the expected MW in CDC42 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab215810 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes : HRP Anti-CDC42 antibody [EPR15620] (ab215810) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Spleen (Mouse) Tissue Lysate at 10 µg
Lane 5 : Spleen (Rat) Tissue Lysate at 10 µg
Lane 6 : HAP1 WT at 20 µg
Lane 7 : CDC42 knockout HAP1 at 20 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 21 kDa
Observed band size: 21 kDa


Exposure time: 30 seconds

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215810 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.