Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human p75 NGF Receptor protein (Fc Chimera)
See all p75 NGF Receptor proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVS ATEPCKPC TECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACR VCEAGSGLVFSCQDKQN TVCEECPDGTYSDEANHVDPCLPCTVCEDTE RQLRECTRWADAECEEIPGRWITRSTP PEGSDSTAPSTQEPEAPPEQD LIASTVAGVVTTVMGIPKVDKKVEPKSCDKTHTCPPCP APELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISK AKGQPREPQV YTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLSLSPGK -
Amino acids
1 to 237 -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
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ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
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Functional Studies - Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)Densitometry of protein isoforms visualised by 2-D. The triangle indicates the theoretical MW and pI of the protein.
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SDS-PAGE - Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)1D SDS-PAGE of ab83679 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: MW markers; Lane 2: ab83679; Lane 3: ab83679 treated with PNGase F to remove potential N-linked glycans; Lane 4: ab83679 treated with a glycosidase cocktail to remove potential N and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates the presence of Olinked glycans. Faint bands in lane 3 and lane 4 are glycosidase enzymes.
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SDS-PAGE - Recombinant Human p75 NGF Receptor protein (Fc Chimera) (ab83679)A sample of ab83679 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
