Anti-GFR alpha 3/GFRA3 antibody (ab205361)
Key features and details
- Chicken polyclonal to GFR alpha 3/GFRA3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-GFR alpha 3/GFRA3 antibody
See all GFR alpha 3/GFRA3 primary antibodies -
Description
Chicken polyclonal to GFR alpha 3/GFRA3 -
Host species
Chicken -
Specificity
ab205361 doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4 -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human GFR alpha 3/GFRA3. Produced using CHO-based proprietary suspension cell line. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: O60609 -
Positive control
- U2OS cells; His tagged GFR alpha 3/GFRA3 protein.
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General notes
This product was previously labelled as GFR alpha 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GFR alpha 3/GFRA3 antibody (ab205361)
Immunofluorescent analysis of U2OS cells labeling GFR alpha 3/GFRA3 using ab205361 at 1/3000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
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Western blot - Anti-GFR alpha 3/GFRA3 antibody (ab205361)Anti-GFR alpha 3/GFRA3 antibody (ab205361) at 1/5000 dilution + His tagged GFR alpha 3/GFRA3 protein at 0.1 µg
Secondary
Rabbit anti-chicken antibody
Predicted band size: 44 kDa
