Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)
Key features and details
- Rabbit polyclonal to Dnmt3a (phospho S390 + S393)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dnmt3a (phospho S390 + S393) antibody
See all Dnmt3a primary antibodies -
Description
Rabbit polyclonal to Dnmt3a (phospho S390 + S393) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
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Positive control
- This antibody gave a positive signal in HEK293 whole cell lysate. This antibody gave a positive signal in the following methanol fixed cell lines: HepG2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
Tissue specificity
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
Cellular localization
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 359716 Cow
- Entrez Gene: 482996 Dog
- Entrez Gene: 1788 Human
- Entrez Gene: 444984 Rat
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- SwissProt: Q1LZ53 Rat
- Unigene: 515840 Human
see all -
Alternative names
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
Images
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Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)All lanes : Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763) at 1 µg/ml
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 + S393 peptide at 1 µg/ml
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 peptide at 1 µg/ml
Lane 4 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S393 peptide at 1 µg/ml
Lane 5 : HEK293 (Human) Whole Cell Lysate (ab52256) with Non modified control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)Lysates from U2OS WT cells were loaded as input. The western blot was probed with anti Dnmt3a (phospho S390 + S393) antibody (ab87763) either in the presence or absence of double modified peptide phosphorylated on S390 and S393 as indicated in the figure. Detected phospho Dnmt3a is indicated by the arrow. Please note: the observed band migrates at a higher MW than predicted probably because of phosphorylation. This data was provided by an anonymous collaborator. -
Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)ICC/IF image of ab87763 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87763 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (2)
ab87763 has been referenced in 2 publications.
- Fan L et al. Enhancement of the chondrogenic differentiation of mesenchymal stem cells and cartilage repair by ghrelin. J Orthop Res N/A:N/A (2019). PubMed: 30644571
- Deplus R et al. Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a. Cell Rep 8:743-53 (2014). WB ; Mouse, Human . PubMed: 25066127
Images
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Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)All lanes : Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763) at 1 µg/ml
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 + S393 peptide at 1 µg/ml
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S390 peptide at 1 µg/ml
Lane 4 : HEK293 (Human) Whole Cell Lysate (ab52256) with Phospho S393 peptide at 1 µg/ml
Lane 5 : HEK293 (Human) Whole Cell Lysate (ab52256) with Non modified control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Western blot - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)Lysates from U2OS WT cells were loaded as input. The western blot was probed with anti Dnmt3a (phospho S390 + S393) antibody (ab87763) either in the presence or absence of double modified peptide phosphorylated on S390 and S393 as indicated in the figure. Detected phospho Dnmt3a is indicated by the arrow. Please note: the observed band migrates at a higher MW than predicted probably because of phosphorylation. This data was provided by an anonymous collaborator.
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Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a (phospho S390 + S393) antibody (ab87763)
ICC/IF image of ab87763 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87763 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
