Mouse MEK3 Antibody Pair - BSA and Azide free (ab241703)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse MEK3 Antibody Pair - BSA and Azide free
See all MEK3 kits -
Assay type
ELISA set -
Range
250 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify MEK3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab217775), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse MEK3 Capture Antibody (unconjugated) 1 x 100µg Mouse MEK3 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Involvement in disease
Note=Defects in MAP2K3 may be involved in colon cancer. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Alternative names
- AW212142
- dual specificity mitogen activated protein kinase kinase 3
- Dual specificity mitogen-activated protein kinase kinase 3
see all -
Database links
- Entrez Gene: 26397 Mouse
- SwissProt: O09110 Mouse
- Unigene: 18494 Mouse
Images
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Sandwich ELISA - Mouse MEK3 Antibody Pair - BSA and Azide free (ab241703)To learn more about the advantages of recombinant antibodies see here.
