Mouse MEK3 Matched Antibody Pair Kit (ab221441)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 250 pg/ml - 8000 pg/ml
Overview
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Product name
Mouse MEK3 Matched Antibody Pair Kit
See all MEK3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
9 pg/ml -
Range
250 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MEK3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MEK3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse MEK3 Capture Antibody 2 x 50µg 1 x 50µg Mouse MEK3 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse MEK3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Involvement in disease
Note=Defects in MAP2K3 may be involved in colon cancer. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Alternative names
- AW212142
- dual specificity mitogen activated protein kinase kinase 3
- Dual specificity mitogen-activated protein kinase kinase 3
see all -
Database links
- Entrez Gene: 26397 Mouse
- SwissProt: O09110 Mouse
- Unigene: 18494 Mouse
Images
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Mouse MEK3 standard curve
Standard calibration curve. Background substracted values are graphed.
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Sandwich ELISA - Mouse MEK3 Matched Antibody Pair Kit (ab221441)To learn more about the advantages of recombinant antibodies see here.
