Anti-NMT2 antibody (ab230028)
Key features and details
- Rabbit polyclonal to NMT2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NMT2 antibody
See all NMT2 primary antibodies -
Description
Rabbit polyclonal to NMT2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human NMT2 aa 1-150.
Database link: O60551 -
Positive control
- WB: Human placenta tissue lysate; K562, A431 and HepG2 whole cell lysate. IHC-P: Human testis and prostate tissue. IP: HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMT2 antibody (ab230028)All lanes : Anti-NMT2 antibody (ab230028) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (ab230028)Paraffin-embedded human testis tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (ab230028)Paraffin-embedded human prostate tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-NMT2 antibody (ab230028)NMT2 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate using ab230028.
Lane 1: Control IgG IP in HepG2 whole cell lysate.
Lane 2: ab230028 in HepG2 whole cell lysate.
Lane 3: NMT2 in HepG2 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50,000).
