Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Arginine deiminase protein (ab119147)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Arginine deiminase protein
    See all Arginine deiminase proteins and peptides

  • Biological activity

    Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.

  • Purity

    > 95 % SDS-PAGE.
    Purity greater than 97% by SDS-PAGE gel and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P23793

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mycoplasma arginini

    • Sequence

      SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILES HDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLED SEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVD PMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTP WYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKEC EFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVN GGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGT NYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSM PLSRKDVKW

    • Predicted molecular weight

      46 kDa

    • Amino acids

      2 to 410

    • Specifications

    Our Abpromise guarantee covers the use of ab119147 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized

    • Additional notes


      Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Constituents: 0.003% DTT, 0.03% Sodium phosphate

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute in water to a concentration of 0.1-1.0 mg/ml.

    General Info

    • Alternative names

      • AD
      • ADI
      • Arginine deiminase
      • Arginine dihydrolase
      • EC=3.5.3.6
      see all

    • Relevance

      Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2.

    • Cellular localization

      Cytoplasmic

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab119147? Please let us know so that we can cite the reference in this datasheet.

ab119147 has not yet been referenced specifically in any publications.

Preparation and Storage

  • AD
  • ADI
  • Arginine deiminase

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