Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Arginine deiminase protein (ab204629)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 97% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies, HPLC

Description

  • Product name

    Recombinant Arginine deiminase protein
    See all Arginine deiminase proteins and peptides

  • Biological activity

    Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.

  • Purity

    > 97 % SDS-PAGE.
    >97% by HPLC.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P23793

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mycoplasma arginini

    • Sequence

      SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILES HDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLED SEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVD PMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTP WYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKEC EFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVN GGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGT NYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSM PLSRKDVKW

    • Predicted molecular weight

      46 kDa

    • Amino acids

      2 to 410

    • Additional sequence information

      Mycoplasma arginini. CAA38080.1.

    • Specifications

    Our Abpromise guarantee covers the use of ab204629 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

      Constituents: 0.03278% Sodium phosphate, 0.00308% DTT

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

    General Info

    • Alternative names

      • AD
      • ADI
      • Arginine deiminase
      • Arginine dihydrolase
      • EC=3.5.3.6
      see all

    • Relevance

      Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2.

    • Cellular localization

      Cytoplasmic

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab204629? Please let us know so that we can cite the reference in this datasheet.

ab204629 has not yet been referenced specifically in any publications.

Preparation and Storage

  • AD
  • ADI
  • Arginine deiminase

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Recombinant Arginine deiminase protein (ab204629)

Sign up