Anti-MEF2A (phospho T312) antibody (ab30644)
Key features and details
- Rabbit polyclonal to MEF2A (phospho T312)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2A (phospho T312) antibody
See all MEF2A primary antibodies -
Description
Rabbit polyclonal to MEF2A (phospho T312) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic phosphopeptide derived from human MEF2A around the phosphorylation site of Threonine 312.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEF2A (phospho T312) antibody (ab30644) This image is courtesy of an anonymous AbreviewAnti-MEF2A (phospho T312) antibody (ab30644) at 1/1000 dilution + Mouse liver tissue lysate - whole at 40 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A (phospho T312) antibody (ab30644)Immunohistochemical analysis of paraffin-embedded human breast carcinoma. Left: using ab30644 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
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Western blot - Anti-MEF2A (phospho T312) antibody (ab30644)All lanes : Anti-MEF2A (phospho T312) antibody (ab30644) at 1 µg/ml
Lane 1 : Untreated NIH/3T3 cells (5-30ug).
Lane 2 : NIH/3T3 cells treated with PMA (5-30ug)
Predicted band size: 55 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
