Recombinant human Brk/PTK6 protein (Tagged) (ab271391)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Brk/PTK6 protein (Tagged)
See all Brk/PTK6 proteins and peptides -
Biological activity
Specific Activity: 88 pmol/min/µg
Assay conditions: Enzyme reaction was conducted in a buffer (25 µl) containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 µM ATP, 0.2 mg/ml poly-(Glu, Tyr), and BRK for 40 min at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform.
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Purity
>= 65 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLD EAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGA FLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPEL VNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGY FGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALY AVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMC YLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPY KWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVD AGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT -
Predicted molecular weight
80 kDa including tags -
Amino acids
2 to 451 -
Tags
GST tag N-Terminus
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Preparation and Storage
- Breast tumor kinase
- Breast tumour kinase
- Protein tyrosine kinase 6
Images
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SDS-PAGE - (ab271391)SDS-PAGE analysis of ab271391.
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Functional Studies - (ab271391)Specific activity of ab271391.