Human Oncostatin M/OSM ELISA Kit (ab215543)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.1 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Oncostatin M/OSM ELISA Kit
See all Oncostatin M/OSM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 8% Inter-assay Sample n Mean SD CV% Overall 3 7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.1 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 86% - 97% Cell culture media 102 88% - 114% Hep Plasma 95 89% - 98% EDTA Plasma 85 82% - 90% Cit plasma 80 78% - 81% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Oncostatin M/OSM ELISA Kit (ab215543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oncostatin M/OSM protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Oncostatin M/OSM with 2.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Oncostatin M is a 28-kDa pleiotropic cytokine of the IL-6 family that is a product of activated T lymphocytes, monocytes, neutrophils, and some tumor cells including breast cancer epithelial cells. Oncostatin M participates in a number of developmental, skeletal and immunological processes. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. Oncostatin M regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. It uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Oncostatin M is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Oncostatin M Capture Antibody 1 x 600µl 10X Human Oncostatin M Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Oncostatin M Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MGC20461
- ONCM_HUMAN
- Oncostatin M
see all -
Database links
- Entrez Gene: 5008 Human
- Omim: 165095 Human
- SwissProt: P13725 Human
- Unigene: 248156 Human
Images
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Other - Human Oncostatin M/OSM ELISA Kit (ab215543)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Oncostatin M standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Oncostatin M in human cell culture supernatant samples.The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated U937 supernatant 50% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean (target) concentration was determined to be 362.5 pg/mL in neat stimulated U937 supernatant and 2800 pg/mL in neat stimulated PBMC supernatant.
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Interpolated concentrations of spike Oncostatin M in human serum and plasma samples.The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Comparison of Oncostatin M in unstimulated and TPA stimulated human U937 cell supernatants.U937 cells were cultured in the absence or presence of 10 ng/mL TPA for 72 hours. The concentrations of Oncostatin M were measured in 50% supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 363 pg/mL in neat TPA stimulated U937 cell supernatant, 24.9 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).
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Comparison of Oncostatin M in unstimulated and PHA-M stimulated human PBMC cell supernatants.Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Oncostatin M were measured in 25% supernatant samples in duplicates and interpolated from the Oncostatin M standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 2800 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 96 pg/mL in neat unstimulated supernatants and undetectable in media (not shown).
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Sandwich ELISA - Human Oncostatin M/OSM ELISA Kit (ab215543)To learn more about the advantages of recombinant antibodies see here.
