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Epigenetics and Nuclear Signaling DNA methylation DNMTs

Anti-Dnmt3a antibody - N-terminal (ab228691)

Anti-Dnmt3a antibody - N-terminal (ab228691)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Dnmt3a - N-terminal
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Anti-Dnmt3a antibody - N-terminal (ab227725)

Overview

  • Product name

    Anti-Dnmt3a antibody - N-terminal
    See all Dnmt3a primary antibodies
  • Description

    Rabbit polyclonal to Dnmt3a - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human Dnmt3a (N terminal). The exact sequence is proprietary.
    Database link: Q9Y6K1

  • Positive control

    • WB: Dnmt3a shRNA transfected HEK-293T, Neuro-2A, PC-12, Rat2 and HEK-293T whole cell extracts. IP: HEK-293T nuclear and whole cell extracts. ICC/IF: A431 cells. IHC-P: Mouse ovary tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

Images

  • Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    All lanes : Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 102 kDa



    5% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Immunocytochemistry/ Immunofluorescence - Anti-Dnmt3a antibody - N-terminal (ab228691)

    A431 cells (human epidermoid carcinoma cell line) cells stained for Dnmt3a (green) using ab228691 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt3a antibody - N-terminal (ab228691)

    Paraffin-embedded mouse ovary tissue stained for Dnmt3a using ab228691 at 1/500 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)

    Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts with 5 μg of ab228691. Western blot was performed from the immunoprecipitate using ab228691.

    Lane 1: HEK-293T whole cell extract (Input).
    Lane 2: Control IgG.
    Lane 3: ab228691 IP in HEK-293T whole cell extract.

  • Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    All lanes : Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (Rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 102 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/1000 dilution + Neuro2A (Mouse neuroblastoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 102 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Immunoprecipitation - Anti-Dnmt3a antibody - N-terminal (ab228691)

    Dnmt3a was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract with 4 µg of ab228691. Western blot was performed form the immunoprecipitate using ab228691 at 1/2000 dilution.
    Lane 1: HEK-293T nuclear lysate 50 µg 
    Lane 2: 4 µg of preimmune Rabbit IgG instead of ab228691 in HEK-293T nuclear extract.
    Lane 3: ab228691 IP in HEK-293T nuclear extract.

    5% SDS-PAGE gel.

  • Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    Western blot - Anti-Dnmt3a antibody - N-terminal (ab228691)
    All lanes : Anti-Dnmt3a antibody - N-terminal (ab228691) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cells extract
    Lanes 2-3 : Dnmt3a shRNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 102 kDa



    5% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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