Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4398] to MGMT - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MGMT antibody [EPR4398] - BSA and Azide free
    See all MGMT primary antibodies

  • Description

    Rabbit monoclonal [EPR4398] to MGMT - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247735 is the carrier-free version of ab108989 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247735 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)

    This data was developed using ab108989, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: MGMT knockout HAP1 cell lysate (20 µg)

    Lane 3: MCF7 cell lysate (20 µg)

    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108989 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108989 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108989 and ab8245 (loading control to MGMT) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    All lanes : Anti-MGMT antibody [EPR4398] (ab108989) at 1/1000 dilution (purified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108989, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    Immunoprecipitation - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    This data was developed using ab108989, the same antibody clone in a different buffer formulation.ab108989 (purified) at 1/30 immunoprecipitating MGMT in 10 μg MCF7 (Lanes 1 and 2, observed at 23 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    Western blot - Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    All lanes : Anti-MGMT antibody [EPR4398] (ab108989) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    This data was developed using ab108989, the same antibody clone in a different buffer formulation.

  • Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)
    Anti-MGMT antibody [EPR4398] - BSA and Azide free (ab247735)

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