Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23627-50] to A2BP1/Fox1/RBFOX1 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, Flow Cyt, IP, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free
    See all A2BP1/Fox1/RBFOX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23627-50] to A2BP1/Fox1/RBFOX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    IHC-Fr
    Rat
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain; Mouse brain, brain cortex and skeletal muscle; Rat brain and brain cortex, U-2 OS; Neuro-2a; L6 lysates. IHC-Fr: Mouse cerebrum; Rat cerebrum. ICC: Rat primary neural/glia; Neuro-2a; L6; U-2 OS cells; mouse primary neural/glia cells. Flow Cyt: U-2 OS; Neuro-2a cells. IP: Human brain; Mouse tissue lysates.

  • General notes

    ab278528 is the carrier-free version of ab254412. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    All lanes : Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse brain cortex tissue lysate
    Lane 5 : Mouse skeletal muscle tissue lysate
    Lane 6 : Mouse liver tissue lysate
    Lane 7 : Rat brain tissue lysate
    Lane 8 : Rat brain cortex tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 42 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: liver(PMID:12574126, PMID:16260614, PMID:20724578, PMID:20724578, PMID:10814712).

    Exposure time: Lanes 1-2: 3 minutes; Lanes 3-6: 3 seconds; Lanes 7-8: 6 seconds.

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor®594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution.

  • Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    A2BP1/Fox1/RBFOX1 was immunoprecipitated from 0.35 mg Human brain tissue lysate 10 ug with ab254412 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10 ug

    Lane 2: ab254412 IP in Human brain tissue lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab254412 in human brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

     

  • Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Western blot - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    All lanes : Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] (ab254412) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 3 : L6 (rat skeletal muscle myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 42 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 103 seconds.

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) antibody at 1/1000 (Green). Confocal image showing nuclear and cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 (1.144 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Flow Cytometry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunoprecipitation - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    A2BP1/Fox1/RBFOX1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab254412 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab254412 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254412 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (11.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/500 (1.144 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunocytochemistry - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L6 cells labelling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on rat liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)
    Immunohistochemistry (Frozen sections) - Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23627-50] - BSA and Azide free (ab278528)

    This data was developed using ab254412, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling A2BP1/Fox1/RBFOX1 with ab254412 at 1/100 (5.72 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on mouse liver (PMID: 16260614) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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