Anti-TRA2A antibody (ab137248)
Key features and details
- Rabbit polyclonal to TRA2A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRA2A antibody
See all TRA2A primary antibodies -
Description
Rabbit polyclonal to TRA2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human TRA2A (NP_037425.1).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab137248 was affinity purified using an epitope specific to TRA2A immobilized on solid support . -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-TRA2A antibody (ab137248)
ab137248 immunoprecipitating TRA2A in HeLa (human cervix adenocarcinoma) whole cell lysate.
Lane 1: ab137248 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 2: ab138448 at 6 μg/mg of lysate + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 3 (-): Control IgG + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
For western blotting, ab138448 at 0.4 μg/mL
Detection: Chemiluminescence with exposure time of 10 seconds.
ab137248 recognises the upstream epitope.
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Immunoprecipitation - Anti-TRA2A antibody (ab137248)Immunoprecipitation with ab137248 at 6µg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Subsequent WB detection used an anti-TRA2A antibody which recognizes a downstream epitope at 0.4µg/ml. Detected by chemiluminescence with exposure time of 10 seconds.
