Anti-TRA2A antibody (ab72625)
Key features and details
- Rabbit polyclonal to TRA2A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRA2A antibody
See all TRA2A primary antibodies -
Description
Rabbit polyclonal to TRA2A -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total TRA2A protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from an internal region of human TRA2A.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TRA2A antibody (ab72625) at 1/250 dilution
Lanes 1 & 3 & 5 : 293T whole cell lysate
Lanes 2 & 4 & 6 : Mouse ES (129Sv) whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 27 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 1 minute
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All lanes : Anti-TRA2A antibody (ab72625) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 27 kDa, 44 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1:50 dilution.
Left image un-treated.Right image treated with immunizing peptide. -
Immunofluorescence analysis of Hela cells using ab72625 at a 1:500 dilution.
Left image un-treated.Right image treated with immunizing peptide.