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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Anti-TRA2A antibody (ab72625)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-TRA2A antibody (ab72625)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TRA2A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TRA2A antibody
    See all TRA2A primary antibodies
  • Description

    Rabbit polyclonal to TRA2A
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total TRA2A protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal region of human TRA2A.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-TRA2A antibody (ab72625)
    Western blot - Anti-TRA2A antibody (ab72625) This image is courtesy of an anonymous Abreview
    All lanes : Anti-TRA2A antibody (ab72625) at 1/250 dilution

    Lanes 1 & 3 & 5 : 293T whole cell lysate
    Lanes 2 & 4 & 6 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 27 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-TRA2A antibody (ab72625)
    Western blot - Anti-TRA2A antibody (ab72625)
    All lanes : Anti-TRA2A antibody (ab72625) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 27 kDa, 44 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (ab72625)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (ab72625)
    Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1:50 dilution.
    Left image un-treated.Right image treated with immunizing peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (ab72625)
    Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (ab72625)
    Immunofluorescence analysis of Hela cells using ab72625 at a 1:500 dilution.
    Left image un-treated.Right image treated with immunizing peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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