Anti-TRA2A antibody (ab138448)
Key features and details
- Rabbit polyclonal to TRA2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRA2A antibody
See all TRA2A primary antibodies -
Description
Rabbit polyclonal to TRA2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between amino acids 1 and 50 of Human TRA2A (NP_037425.1).
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Positive control
- HeLa, 293T and Jurkat cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRA2A antibody (ab138448)All lanes : Anti-TRA2A antibody (ab138448) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-TRA2A antibody (ab138448)ab138448 at 6 μg/mg of lysate immunoprecipitating TRA2A in HeLa (human cervix adenocarcinoma) whole cell lysate.
Lane 1: ab137248 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 2: ab138448 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 3 (-): Control IgG + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
For western blotting, ab138448 at 0.4 μg/mL
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunoprecipitation - Anti-TRA2A antibody (ab138448)Immunoprecipitation detection of TRA2A in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138448 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138448 at 0.4µg/ml. Chemiluminescence with an exposure time of 30 seconds.
