Anti-HNRPA3/HNRNPA3 antibody (ab78300)
Key features and details
- Rabbit polyclonal to HNRPA3/HNRNPA3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HNRPA3/HNRNPA3 antibody -
Description
Rabbit polyclonal to HNRPA3/HNRNPA3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human HNRPA3/HNRNPA3 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90217) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Raji; SHSY-5Y; Caco 2.
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General notes
This product was previously labelled as HNRPA3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab78300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Target
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Relevance
HNRPA3 plays a role in cytoplasmic trafficking of RNA. It binds to the cis-acting response element(A2RE) and may be involved in pre-mRNA splicing. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 220988 Human
- Entrez Gene: 229279 Mouse
- Entrez Gene: 362152 Rat
- Omim: 605372 Human
- SwissProt: P51991 Human
- SwissProt: Q8BG05 Mouse
- SwissProt: Q6URK4 Rat
- Unigene: 516539 Human
see all -
Alternative names
- FBRNP antibody
- Heterogeneous nuclear ribonucleoprotein A3 antibody
- HNRPA3 antibody
Images
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Western blot - Anti-HNRPA3/HNRNPA3 antibody (ab78300)All lanes : Anti-HNRPA3/HNRNPA3 antibody (ab78300) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteHNRPA3/HNRNPA3 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPA3/HNRNPA3 antibody (ab78300)IHC image of ab78300 staining in Human Cervical Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78300, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab78300 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HNRPA3/HNRNPA3 antibody (ab78300)All lanes : Anti-HNRPA3/HNRNPA3 antibody (ab78300) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteHNRPA3/HNRNPA3 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPA3/HNRNPA3 antibody (ab78300)IHC image of ab78300 staining in Human Cervical Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78300, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.