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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing

Human SRSF6 (SR protein repeat) knockout HEK-293T cell lysate (ab258210)

Price and availability

331 689 ₸

Availability

Order now and get it on Friday March 05, 2021

Human SRSF6 (SR protein repeat) knockout HEK-293T cell lysate (ab258210)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human SRSF6 (SR protein repeat) knockout HEK-293T cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261172 - Human SRSF6 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.
  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q13247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • arginine/serine-rich 6
    • B52
    • Epididymis secretory protein Li 91
    • fc17h09
    • HEL S 91
    • MGC128807
    • MGC5045
    • Pre mRNA splicing factor SRP55
    • Pre-mRNA-splicing factor SRP55
    • Serine/arginine-rich splicing factor 6
    • SFRS6
    • Splicing factor
    • Splicing factor arginine/serine rich 55 kDa
    • Splicing factor arginine/serine rich 6
    • SR splicing factor 6
    • SRP55
    • SRSF6
    • SRSF6_HUMAN
    • wu:faa54g02
    • wu:fc17h09
    • zgc:103497
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261172 - Human SRSF6 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Western blot - Human SRSF6 (SR protein repeat) knockout HEK293T cell lysate (ab258210)
    Western blot - Human SRSF6 (SR protein repeat) knockout HEK293T cell lysate (ab258210)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:SRSF6 knockout HEK293T cell lysate (20 ug)
    Lane 3:Jurkat cell lysate (20 ug)
    Lane 4:K-562 cell lysate (20 ug)

    ab140623 was shown to specifically react with SR protein repeat/SRSF6 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266529 (knockout cell lysate ab258210) was used. Wild-type and SR protein repeat/SRSF6 knockout samples were subjected to SDS-PAGE. ab140623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human SRSF6 knockout HEK293T cell lysate (ab258210)
    Sanger Sequencing - Human SRSF6 knockout HEK293T cell lysate (ab258210)
    Homozygous: 1 bp insertion in exon 1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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