Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)
Key features and details
- Rabbit polyclonal to 14-3-3 beta + zeta (phospho S184 + S186)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody -
Description
Rabbit polyclonal to 14-3-3 beta + zeta (phospho S184 + S186) -
Host species
Rabbit -
Specificity
ab111856 detects endogenous levels of 14-3-3 beta + zeta only when phosphorylated at serine 184 or serine 186. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from Human 14-3-3 beta + zeta around the phosphorylation site of serine 184 or serine 186 (L-N-SP-P-E).
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Positive control
- RAW264.7 cell extracts, treated with UV (15mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111856 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856)All lanes : Anti-14-3-3 beta + zeta (phospho S184 + S186) antibody (ab111856) at 1/500 dilution
Lane 1 : RAW264.7 cell extracts, treated with UV (15mins)
Lane 2 : RAW264.7 cell extracts, treated with UV (15mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa