Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22723-24] to SEC23B - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free
    See all SEC23B primary antibodies

  • Description

    Rabbit monoclonal [EPR22723-24] to SEC23B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LIF/His-tagged SEC23B recombinant protein. MEF transfected with scrambled siRNA control whole cell lysate. MEF transfected with siRNA specifically targeting SEC23B whole cell lysate. MEF, RAW264.7 and NIH/3T3 whole cell lysate. Mouse liver tissue lysate. IHC-P: Mouse liver tissue. ICC/IF: MEF cells. Flow Cyt: MEF cells. IP: MEF whole cell lysate.

  • General notes

    ab270756 is the carrier-free version of ab245212. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)
    Immunoprecipitation - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

    SEC23B was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 20 ug with ab245212 at 1/30. Western blot was performed on the immunoprecipitate using ab245212 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: MEF whole cell lysate 20 ug.

    Lane 2: ab245212 IP in MEF whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245212 in MEF whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245212).

  • Flow Cytometry - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)
    Flow Cytometry - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (Mouse embryonic fibroblast (immortalized)) cells labeling SEC23B with ab245212 at 1/500 dilution (0.1µg)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) / (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245212).

  • Immunocytochemistry/ Immunofluorescence - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)
    Immunocytochemistry/ Immunofluorescence - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MEF cells labeling SEC23B with ab245212 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocol image showing mainly cytoplasmic staining in MEFs. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245212).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B with 245212 at 1/1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver is observed (PMID: 22745161).
    The section was incubated with ab245212 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245212).

  • Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)
    Anti-SEC23B antibody [EPR22723-24] - BSA and Azide free (ab270756)

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