Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)
![Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.com/ps/products/109/ab109385/Images/ab109385-122177-anti-thioredoxin-trx-antibody-epr6111-western-blot.jpg "Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6111] to Thioredoxin / TRX
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Thioredoxin / TRX antibody [EPR6111]
See all Thioredoxin / TRX primary antibodies -
Description
Rabbit monoclonal [EPR6111] to Thioredoxin / TRX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7, THP-1, HeLa and HepG2 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.03 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6111 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)All lanes : Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa
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![Flow Cytometry - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.com/ps/products/109/ab109385/Images/ab109385-238342-anti-thioredoxin-trx-antibody-epr6111-flow-cytometry.jpg)
Flow Cytometry - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)Overlay histogram showing HeLa cells stained with ab109385 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109385, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.com/ps/products/109/ab109385/Images/ab109385-122176-anti-thioredoxin-trx-antibody-epr6111.jpg)
OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) -
![Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)](https://www.abcam.com/ps/products/109/ab109385/Images/ab109385-4-benefits-of-recombinant-antibodies.png)
Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)
