Anti-Thioredoxin / TRX antibody (ab26320)
Key features and details
- Rabbit polyclonal to Thioredoxin / TRX
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Thioredoxin / TRX antibody
See all Thioredoxin / TRX primary antibodies -
Description
Rabbit polyclonal to Thioredoxin / TRX -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Cat, Pig
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Thioredoxin/ TRX.
Read Abcam's proprietary immunogen policy (Peptide available as ab27815.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). Target
-
Function
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). -
Sequence similarities
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
Post-translational
modificationsIn the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. -
Cellular localization
Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7295 Human
- Entrez Gene: 22166 Mouse
- Entrez Gene: 116484 Rat
- Omim: 187700 Human
- SwissProt: P10599 Human
- SwissProt: P10639 Mouse
- SwissProt: P11232 Rat
- Unigene: 435136 Human
see all -
Alternative names
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
see all
Images
-
Western blot - Anti-Thioredoxin / TRX antibody (ab26320)Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml +
Recombinant human Thioredoxin / TRX protein (Active) (ab51064) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320)Treutlein G et al., 2012, 7(12): e51391, Fig 4.; doi: 10.1371/journal.pone.0051391 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab26320 staining Thioredoxin (red) in Cat urinary bladder tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were blocked with 1% BSA in TBS-T for 40 minutes at room temperature and heat antigen retrieval was processed at 99°C for 15 minutes in 0.1M EDTA-NaOH buffer. Samples were incubated with primary antibody (1/100) overnight at 4°C. Nuclei stained with DAPI (blue). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Muscle tunic
-
Immunoprecipitation - Anti-Thioredoxin / TRX antibody (ab26320)Thioredoxin / TRX was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Thioredoxin / TRX and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab26320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 12kDa; Thioredoxin / TRX
-
Western blot - Anti-Thioredoxin / TRX antibody (ab26320)Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaab26320 detects a band of 12 kDa in HeLa, Jurkat and A431 whole cell lysates. The data for the Jurkat and A431 lysates is not shown.
-
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320)This image is courtesy of an Abreview submitted by Dr Kirk McManusab26320, at 1/200 dilution, staining human TRX in assynchronous HeLa cells by ICC/IF. -
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320)This image is courtesy of an abreview submitted by Dr David Tonge, Wolfson CARD, Kings College LondonImmunofluorescent detection of Thioredoxin /TRX using antibody ab26320. Chinese Hamster Cell (Ovary cells) were fixed in formaldehyde and permeabilized in 0.1% Triton X in PBS/3% BSA. Primary antibody was incubated at 1/1000 for 1h @ 4°C in PBS containing 3% BSA. Secondary antibody: anti-rabbit conjugated to Alexa Fluor® 568 used @ 1/300. CHO cells co-transfected with plasmids encoding GFP and a mutated form of human thioredoxin which does not enter the nucleus, and labelled using ab26320 (1/1000) and Alexa 568 (1/300). Panel [A] demonstrates GFP immunoreactivity. [B ]thioredoxin immunoreactity; many of the same cells are immunoreactive for both GFP and thioredoxin. Panel [D] thioredoxin primary antibody was omitted, hence there is no immunoeactivity.
Protocols
Datasheets and documents
References (27)
ab26320 has been referenced in 27 publications.
- Ji YJ et al. Down-regulation of DJ-1 Augments Neuroinflammation via Nrf2/Trx1/NLRP3 Axis in MPTP-induced Parkinson's Disease Mouse Model. Neuroscience N/A:N/A (2020). PubMed: 32526245
- Dang R et al. Predictable chronic mild stress promotes recovery from LPS-induced depression. Mol Brain 12:42 (2019). PubMed: 31053149
- Fei J et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757
- Wang W et al. Pinellia pedatisecta lectin exerts a proinflammatory activity correlated with ROS-MAPKs/NF-?B pathways and the NLRP3 inflammasome in RAW264.7 cells accompanied by cell pyroptosis. Int Immunopharmacol 66:1-12 (2019). PubMed: 30415189
- Ji Cho M et al. Oxidative stress-mediated TXNIP loss causes RPE dysfunction. Exp Mol Med 51:1-13 (2019). PubMed: 31615975
Images
-
Western blot - Anti-Thioredoxin / TRX antibody (ab26320)Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml +
Recombinant human Thioredoxin / TRX protein (Active) (ab51064) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320) Treutlein G et al., 2012, 7(12): e51391, Fig 4.; doi: 10.1371/journal.pone.0051391 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ab26320 staining Thioredoxin (red) in Cat urinary bladder tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were blocked with 1% BSA in TBS-T for 40 minutes at room temperature and heat antigen retrieval was processed at 99°C for 15 minutes in 0.1M EDTA-NaOH buffer. Samples were incubated with primary antibody (1/100) overnight at 4°C. Nuclei stained with DAPI (blue). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Muscle tunic
-
Immunoprecipitation - Anti-Thioredoxin / TRX antibody (ab26320)
Thioredoxin / TRX was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Thioredoxin / TRX and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab26320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 12kDa; Thioredoxin / TRX
-
Western blot - Anti-Thioredoxin / TRX antibody (ab26320)Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaab26320 detects a band of 12 kDa in HeLa, Jurkat and A431 whole cell lysates. The data for the Jurkat and A431 lysates is not shown.
-
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320) This image is courtesy of an Abreview submitted by Dr Kirk McManusab26320, at 1/200 dilution, staining human TRX in assynchronous HeLa cells by ICC/IF.
-
Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin / TRX antibody (ab26320) This image is courtesy of an abreview submitted by Dr David Tonge, Wolfson CARD, Kings College London
Immunofluorescent detection of Thioredoxin /TRX using antibody ab26320. Chinese Hamster Cell (Ovary cells) were fixed in formaldehyde and permeabilized in 0.1% Triton X in PBS/3% BSA. Primary antibody was incubated at 1/1000 for 1h @ 4°C in PBS containing 3% BSA. Secondary antibody: anti-rabbit conjugated to Alexa Fluor® 568 used @ 1/300. CHO cells co-transfected with plasmids encoding GFP and a mutated form of human thioredoxin which does not enter the nucleus, and labelled using ab26320 (1/1000) and Alexa 568 (1/300). Panel [A] demonstrates GFP immunoreactivity. [B ]thioredoxin immunoreactity; many of the same cells are immunoreactive for both GFP and thioredoxin. Panel [D] thioredoxin primary antibody was omitted, hence there is no immunoeactivity.
