Anti-TMEM109/Mitsugumin-23 antibody (ab241992)
Key features and details
- Rabbit polyclonal to TMEM109/Mitsugumin-23
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-TMEM109/Mitsugumin-23 antibody
See all TMEM109/Mitsugumin-23 primary antibodies -
Description
Rabbit polyclonal to TMEM109/Mitsugumin-23 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide within Human TMEM109/Mitsugumin-23 aa 25-75. The exact sequence is proprietary.
Database link: Q9BVC6 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: TMEM109/Mitsugumin-23 IP in HEK-293T whole cell lysate.
-
General notes
This product was previously labelled as TMEM109
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TMEM109/Mitsugumin-23 antibody (ab241992)All lanes : Anti-TMEM109/Mitsugumin-23 antibody (ab241992) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (ab241992)TMEM109/Mitsugumin-23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241992 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab241992 at 1/100 dilution.
Lane 1: ab241992 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.
