Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6110] to Thioredoxin / TRX
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-Thioredoxin / TRX antibody [EPR6110]
See all Thioredoxin / TRX primary antibodies -
Description
Rabbit monoclonal [EPR6110] to Thioredoxin / TRX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to residues in the Human Thioredoxin/TRX protein (UniProt ID: P10599).
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Positive control
- WB: MCF7, THP1, HeLa, and HepG2 whole cell lysate (ab7900) IHC-P: Human colon tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.12 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6110 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
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Immunohistochemistry analysis of Paraffin Embedded Human colon tissue labelling Thioredoxin/TRX with ab133524 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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