Anti-Caspase-2 antibody [Y154] (ab32021)
![Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.com/ps/products/32/ab32021/Images/ab32021-253275-anti-caspase-2-antibody-y154-western-blot.jpg "Anti-Caspase-2 antibody [Y154] (ab32021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y154] to Caspase-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-2 antibody [Y154]
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [Y154] to Caspase-2 -
Host species
Rabbit -
Specificity
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Caspase-2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P42575 -
Positive control
- Jurkat cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y154 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.com/ps/products/32/ab32021/Images/ab32021-43662-anti-caspase-2-antibody-y154-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cleavage fragment)
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![Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.com/ps/products/32/ab32021/Images/ab32021-3-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [Y154] (ab32021)
