Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16796] to Caspase-2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free
    See all Caspase-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16796] to Caspase-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240217 is the carrier-free version of ab179520. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240217 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

    This data was developed using ab179520, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Caspase-2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    All lanes : Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours
    Lane 2 : Untreated HeLa whole cell lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours
    Lane 4 : Untreated C6 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 33,48 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 33,48 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

  • Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Western blot - Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    This data was developed using ab179520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The 33KDa band (cleaved caspase 2) contains subunit p18.

  • Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)
    Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Caspase-2 antibody [EPR16796] - BSA and Azide free (ab240217)

© 2021 Astana Biomed Group LLP. All rights reserved.