Anti-Caspase-2 antibody (ab227241)
Key features and details
- Rabbit polyclonal to Caspase-2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-2 antibody
See all Caspase-2 primary antibodies -
Description
Rabbit polyclonal to Caspase-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human Caspase-2 (internal sequence). The exact sequence is proprietary.
Database link: P42575 -
Positive control
- WB: HeLa cell extract; Jurkat, Raji and NCI-H929 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-2 antibody (ab227241)All lanes : Anti-Caspase-2 antibody (ab227241) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Caspase-2 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
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Western blot - Anti-Caspase-2 antibody (ab227241)All lanes : Anti-Caspase-2 antibody (ab227241) at 1/2500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Caspase-2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa10% SDS-PAGE gel.
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Western blot - Anti-Caspase-2 antibody (ab227241)All lanes : Anti-Caspase-2 antibody (ab227241) at 1/5000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa10% SDS-PAGE gel.
