Anti-CD3 epsilon antibody [E272] (ab32186)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E272] to CD3 epsilon
- Suitable for: IHC-P, IP
- Reacts with: Human
Overview
-
Product name
Anti-CD3 epsilon antibody [E272]
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [E272] to CD3 epsilon -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human -
Immunogen
Synthetic peptide within Human CD3 epsilon aa 150-250 (C terminal). The exact sequence is proprietary.
-
Positive control
- IP: Jurkat cell lysate IHC-P: Human lymph node.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
E272 -
Isotype
IgG -
Research areas
Images
-
Ab32186, at a 1/100 dilution, staining CD3 epsilon in paraffin embedded human lymph node tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or PBS (lane 2: negative control), using ab32186 at a 1/50 dilution. The predicted band size of 23 kDa was observed.
-