Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL54] to CD3 epsilon - BSA and Azide free
  • Suitable for: ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free
    See all CD3 epsilon primary antibodies

  • Description

    Rabbit monoclonal [CAL54] to CD3 epsilon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD3 epsilon aa 150-250. The exact sequence is proprietary.
    Database link: P07766

  • Positive control

    • IHC-P: Human tonsil, gastric carcinoma and NSCLC tissue. ICC/IF: Jurkat cells. Flow cyt: Human PBMCs. IP: Human thymus lysate; Jurkat whole cell lysate.

  • General notes

    Ab251594 is the carrier-free version of ab237707. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251594 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab237707 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the infiltrating T lymphocytes in the human gastric carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab237707 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD3 epsilon with ab237707 at 1/55 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    CD3 epsilon was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000  dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input). 
    Lane 2: ab237707 IP in Jurkat whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237707 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Flow Cytometry - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Flow Cytometry - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized human PBMCs (peripheral blood mononuclear cells) labeling CD3 epsilon with ab237707 at 1/500 (Right compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD3 epsilon conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining rabbit IgG (Left) or ab237707 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    CD3 epsilon was immunoprecipitated from 0.35 mg of human thymus lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000  dilution.

    Lane 1: Human thymus lysate 10 μg (Input). 
    Lane 2: ab237707 IP in human thymus lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237707 in human thymus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Formalin-fixed, paraffin-embedded human NSCLC tissue stained for CD3 epsilon using ab237707 at 0.5 µg/mL in immunohistochemcial analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab237707 at 0.5 µg/mL in immunohistochemcial analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237707).

  • Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)
    Anti-CD3 epsilon antibody [CAL54] - BSA and Azide free (ab251594)

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