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Anti-CD38 antibody [HB-7] (ab235118)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HB-7] to CD38
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD38 antibody [HB-7]
    See all CD38 primary antibodies

  • Description

    Mouse monoclonal [HB-7] to CD38

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human CD38. Human BJAB cell line

  • Positive control

    • ICC/IF: Raji cells. Human PBMC.Flow Cyt: Raji cells. Human PBMC.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)
    Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)
    Immunocytochemistry/ Immunofluorescence - Anti-CD38 antibody [HB-7] (ab235118)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD38 with ab235118 at 5.6µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab235118 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)
    Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)

    Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD38 with ab235118 at 1.12µg/ml (red) compared with a Mouse IgG, monoclonal - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG  (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)
    Flow Cytometry - Anti-CD38 antibody [HB-7] (ab235118)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD38 with ab235118 at 1.12µg/ml compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Anti-CD38 antibody [HB-7] (ab235118)
    Anti-CD38 antibody [HB-7] (ab235118)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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