Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD38 antibody [EPR21079] (ab216343)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21079] to CD38
Suitable for: WB, IHC-P
Reacts with: Mouse

Product name

Anti-CD38 antibody [EPR21079]
See all CD38 primary antibodies
Description

Rabbit monoclonal [EPR21079] to CD38
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: P388D1 whole cell lysate; Mouse spleen, lymph node, colon and thymus lysates. IHC-P: Mouse spleen, liver and kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21079
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID: 25078456). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID: 17200192). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID: 10360965). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CD38 antibody [EPR21079] (ab216343)

All lanes : Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
Lane 3 : Mouse lymph node lysate
Lane 4 : Mouse colon lysate
Lane 5 : Mouse thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID: 7774637).
Anti-CD38 antibody [EPR21079] (ab216343)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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