Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD38 antibody [EPR4106] (ab108403)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4106] to CD38
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD38 antibody [EPR4106]
    See all CD38 primary antibodies

  • Description

    Rabbit monoclonal [EPR4106] to CD38

  • Host species

    Rabbit

  • Specificity

    Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    Lane 1 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution
    Lane 2 : Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa



    Buffer and concentration: 5% NFDM/TBST

    Exposure time, Lane 1: 20 seconds, Lane 2: 30 seconds

  • Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution + Human lymph node lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 34 kDa


    Exposure time: 80 seconds


    Buffer and concentration: 5% NFDM/TBST

     

  • Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
    All lanes : Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution (purified)

    Lane 1 : HuT-78 cell lysate
    Lane 2 : Human fetal thymus tissue lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)
    Flow Cytometry - Anti-CD38 antibody [EPR4106] (ab108403)

    Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD38 antibody [EPR4106] (ab108403)
    OI-RD Scanning - Anti-CD38 antibody [EPR4106] (ab108403)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD38 antibody [EPR4106] (ab108403)
    Anti-CD38 antibody [EPR4106] (ab108403)

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