Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR042F] to CD134 / OX40L receptor
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-CD134 / OX40L receptor antibody [BLR042F]
See all CD134 / OX40L receptor primary antibodies
Description

Rabbit monoclonal [BLR042F] to CD134 / OX40L receptor
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human CD134/ OX40L receptor (extracellular).
Database link: P43489
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR042F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

CD134 / OX40L was immunoprecipitated from 1.0 mg MJ whole cell lysate with ab243898 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243898 at 1/1000 dilution.
Lane 1: ab243898 IP in MJ whole cell lysate.

Lane 2: Control IgG in MJ whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

Western blot analysis using ab243898 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (50 µg).
Lane 2: MCF7 whole cell lysate (50 µg).
Lane 3: HepG2 whole cell lysate (50 µg).
Lane 4: A549 whole cell lysate (50 µg).
Lane 5: SW 620 whole cell lysate (50 µg).
Lane 6: SK whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
Lane 8: TCMK-1 whole cell lysate (50 µg).
Lane 9: NIH/3T3 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

Formalin-fixed, paraffin-embedded MJ cells labeling CD134 / OX40L using ab243898 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CD134 / OX40L using ab243898 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-CD134 / OX40L receptor antibody [BLR042F] (ab243898)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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