Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

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Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor
Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-CD134 / OX40L receptor antibody [EPR23000-42]
See all CD134 / OX40L receptor primary antibodies
Description

Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human PBMC treated with 10 µg/ml phytohaemagglutinin (PHA) for 2 days, whole cell lysate; human thymus and lymph node tissue lysates. IHC-P: Human thymus and Hodgkin's lymphoma tissue. IP: Human thymus tissue lysate. Flow cyt: Human peripheral blood mononuclear cell (PBMC) treated with 10 µg/ml phytohemagglutinin (PHA) for 2days. ICC/IF: Human PBMC treated with phytohaemagglutinin (PHA) (10 µg/ml for 2 days).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23000-42
Isotype

IgG
Research areas

Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

All lanes : Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466) at 1/1000 dilution

Lane 1 : Human thymus tissue lysate
Lane 2 : Human lymph node

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 29 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

This blot was developed using a higher sensitivity ECL substrate.

The molecular weight observed is consistent with what has been described in the literature (PMID:9108415, 23897980).
Western blot - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

All lanes : Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466) at 1/1000 dilution

Lane 1 : Untreated human PBMC, whole cell lysate
Lane 2 : Human PBMC treated with 10 µg/ml phytohaemagglutinin (PHA) for 2 days, whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/500 dilution

Predicted band size: 29 kDa
Observed band size: 37,50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 37 seconds.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:9108415,23897980).

Negative control: Jurkat (PMID:9108415).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CD134 / OX40L with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142). Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

CD134 / OX40L receptor was immunoprecipitated from 0.35 mg Human thymus lysate with ab264466 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264466 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Human thymus lysate 10ug

Lane 2: ab264466 IP in Human thymus lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264466 in Human thymus lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

Flow cytometric analysis of 2% Paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10 μg/ml phytohemagglutinin (PHA) for 2days labelling CD132 / OX40L receptor with ab264466 at 1/500 dilution 9Right) (Red) compared with a Rabbit monoclonal IgG (ab172730) control (Left). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD3 conjugated to Alexa Fluor^® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab264466 (Right). (PMID: 9108415,17609274).
Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs labelling CD34 / OX40L with ab264466 at 1/50 (10 ug/ml) dilution, followed by ab150077 AlexaFluo^r®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in human PBMC treated with phytohaemagglutinin (PHA) (10 μg/ml for 2 days) (PMID: 9108415). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
Anti-CD134 / OX40L receptor antibody [EPR23000-42] (ab264466)

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