Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23001-88] to CD134 / OX40L receptor
Suitable for: IP, Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-CD134 / OX40L receptor antibody [EPR23001-88]
See all CD134 / OX40L receptor primary antibodies
Description

Rabbit monoclonal [EPR23001-88] to CD134 / OX40L receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human PBMC treated with 10 µg/ml phytohaemagglutinin (PHA) for 2 days, cell lysate; human thymus tissue and lymph node tissue lysates. IHC-P: Human thymus and Hodgin's lymphoma tissue. IP: Human thymus tissue lysate. Flow cyt: Human PBMCs.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23001-88
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with ab264465 at 1/2000 dilution (0.26 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID: 8547142) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

All lanes : Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465) at 1/1000 dilution

Lane 1 : Untreated human PBMC, whole cell lysate
Lane 2 : Human PBMC treated with 10 µg/ml phytohaemagglutinin (PHA) for 2 days, whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/500 dilution

Predicted band size: 29 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 37 seconds.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:9108415,23897980).

Negative control: Jurkat (PMID:9108415).
Western blot - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

All lanes : Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465) at 1/1000 dilution

Lane 1 : Human thymus tissue lysate
Lane 2 : Human lymph node lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 29 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

This blot was developed using a higher sensitivity ECL substrate.

The molecular weight observed is consistent with what has been described in the literature (PMID:9108415,23897980).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD134 / OX40L receptor with ab264465 at 1/2000 dilution (0.26 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID: 8547142) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

CD134 / OX40L receptor was immunoprecipitated from 0.35 mg Human thymus lysate with ab264465 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264465 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Human thymus lysate

Lane 2: ab264465 IP in Human thymus lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264465 in Human thymus lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml phytohemagglutinin (PHA) for 2 days labelling CD134 / OX40L receptor with ab264465 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab264465 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. (PMID: 9108415,17609274).

Gated on viable cells.
Anti-CD134 / OX40L receptor antibody [EPR23001-88] (ab264465)

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