Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-CD134 / OX40L receptor antibody [EPR17Y]
See all CD134 / OX40L receptor primary antibodies -
Description
Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human CD134/ OX40L receptor aa 1-250. The exact sequence is proprietary.
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Positive control
- WB: Human lymph node, spleen and tonsil tissue lysates and PMBC cell lysate treated with TPA.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17Y -
Isotype
IgG -
Research areas
Images
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Flow cytometry analysis of Human PBMC treated with 1©:g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (Ab98462) was used as the secondary antibody at 1/2000 dilution.
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All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution
Lane 1 : Human lymph node
Lane 2 : Human spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution (unpurified)
Lane 1 : PBMC cell lysates, untreated
Lane 2 : PBMC cell lysates, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Note: the actual molecular weight is larger than the predicted due to glycoslyation. -