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Immunology Adaptive Immunity T Cells CD

Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD134 / OX40L receptor antibody [EPR17Y]
    See all CD134 / OX40L receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD134/ OX40L receptor aa 1-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human lymph node, spleen and tonsil tissue lysates and PMBC cell lysate treated with TPA.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Images

  • Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)

    Flow cytometry analysis of Human PBMC treated with 1&#169:g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (Ab98462) was used as the secondary antibody at 1/2000 dilution.

  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution

    Lane 1 : Human lymph node
    Lane 2 : Human spleen

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution (unpurified)

    Lane 1 : PBMC cell lysates, untreated
    Lane 2 : PBMC cell lysates, treated with TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Note: the actual molecular weight is larger than the predicted due to glycoslyation.
  • Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)
    Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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