Anti-Reelin antibody [EPR22515-75] (ab230820)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22515-75] to Reelin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Reelin antibody [EPR22515-75]
See all Reelin primary antibodies -
Description
Rabbit monoclonal [EPR22515-75] to Reelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse hippocampus tissue. Mouse, human and rat cerebral cortex tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22515-75 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons of mouse cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons of human cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on GABA-containing interneurons rat cerebral cortex (PMID: 17053810, 9501244) is observed. The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Reelin with ab230820 at 1/1000 dilution (5.63 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: No staining on human placenta.
The section was incubated with ab230820 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
-