Anti-Reelin antibody [G10] (ab78540)
![Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.com/ps/products/78/ab78540/Images/ab78540-94334-anti-reelin-antibody-g10-immunohistochemistry.jpg "Anti-Reelin antibody [G10] (ab78540)")
Key features and details
- Mouse monoclonal [G10] to Reelin
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Reelin antibody [G10]
See all Reelin primary antibodies -
Description
Mouse monoclonal [G10] to Reelin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC (PFA fixed) MouseHumanWB MouseRat
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Immunogen
Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin
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Positive control
- Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.242% Tris -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
G10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)
ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.com/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-7.jpg)
Western blot - Anti-Reelin antibody [G10] (ab78540)All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain membrane lysate
Secondary
All lanes : Goat anti-mouse secondary antibody
conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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![Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.com/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-9.jpg)
Immunohistochemistry (PFA fixed) - Anti-Reelin antibody [G10] (ab78540)Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Reelin antibody [G10] (ab78540)](https://www.abcam.com/ps/products/78/ab78540/Images/Reelin-Primary-antibodies-ab78540-6.jpg)
Western blot - Anti-Reelin antibody [G10] (ab78540)Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg
Secondary
Goat anti-mouse secondary
antibody conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDaThis image was generated using the ascites version of the product.
