Anti-PPAR delta antibody [EPR18517] (ab178866)
![Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266341-anti-ppar-delta-antibody-epr18517-western-blot.jpg "Anti-PPAR delta antibody [EPR18517] (ab178866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18517] to PPAR delta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-PPAR delta antibody [EPR18517]
See all PPAR delta primary antibodies -
Description
Rabbit monoclonal [EPR18517] to PPAR delta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB MouseRatHumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PPAR delta full length recombinant protein; Jurkat, K562, HeLa, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain, heart and kidney lysates. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18517 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Human PPAR delta full length recombinant protein
Lane 2 : Human PPAR alpha full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR delta full length recombinant protein contains aa1-441 with a His-Tag.
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![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266340-anti-ppar-delta-antibody-epr18517-western-blot.jpg)
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 15 seconds.
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![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266339-anti-ppar-delta-antibody-epr18517-western-blot.jpg)
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 10 seconds.
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![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266338-anti-ppar-delta-antibody-epr18517-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).
Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab178866 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-5-benefits-of-recombinant-antibodies.png)
Anti-PPAR delta antibody [EPR18517] (ab178866)
